Per Student Pair

• 72-hour old chicken embryos/eggs, in 37 C humidified incubator (7)
• 110 mm diameter glass dishes, lined with cotton (2)
• Scotch Magic Tape (1)
• 20 G needle attached to a syringe (1)
• Fine scissors (2)
• Fine forceps (2)
• 65 mm Syracuse glass dishes (2)
• Chick saline in 45C water bath (100 ml)
• Test tube rack in 45C water bath
• Filter paper "doughnuts" (8)
• Microknife (Tyler, 1994) and/or iris microdissecting scissors (2)
• Test tubes with caps (6)
• Test tube rack on bench top (1)
• 1.5 ml dropping pipettes (6)
• Embryo spoon (1)
• Gooseneck lamp with 100 W bulb (2)
• Large beaker for eggs/embryos after they have been examined
• Dissecting microscope with lighting from above (2)
• Labeling tape and indelible ink pen (1)
• Atlas
• Stop watch or clock with second hand
• Heart models

• Stock solution of 3% caffeine, made with chick saline (500 ml)
• Stock solution of gin, made with chick saline (500 ml)

This page was last modified May 27, 1999.       Send questions or comments to jxm57@psu.edu

Copyright© 1999 Dr. Jacqueline McLaughlin and Dr. Elizabeth R. McCain   All Rights Reserved
This material may not be reproduced without expressed written permission from the authors.

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