Experiment One: The Inheritance of Anthocyanin


The wild-type Brassica rapa plant expresses a water-soluble blue, purple, or red pigment found in the vacuoles of it's stem cells. Plants with even the slightest tint of stem coloration are considered positive for this phenotype. Absence of this pigment yields the mutant variety with clear to white looking stems. In this experiment you will investigate the inheritance of this trait.

Based on Mendelian Laws, you will formulate a Hypothesis concerning the mode of inheritance of the anthocyanin gene. You will be given F1 heterozygous wild-type (anl/ANL) seeds resulting from a cross previously made between a homozygous true-breeding plant that has anthocyanin present (ANL/ANL) and a true-breeding plant that has no anthocyanin(anl/anl). You will plant these seeds, allow them to germinate, cross-pollinate the flowers, then harvest the F2 seeds in order to observe the phenotypic ratios of the F2 generation.

Before you actually perform the planting of seeds, you must write a Protocol which states the Hypothesis that you are testing and reveals the Prediction that you have devised. It is essential that, in your Protocol, you outline the cross for your Prediction and provide expected results in the form of a Punnett square.

Remember, your Protocol is due on exact date will be announced.

Main Page | Introduction and Objectives | Scientific Investigation | Experimental Procedures | Writing Procedures | Mendelian InheritanceMonohybrid and Dihybrid Exercises | Reference | Miscellaneous | Overview of Experiment One | Overview of Experiment Two | Overview of Experiment Three |