Experiment Two: The Inheritance of Variegated and Non-variegated Leaf Color

The wild-type Brassica rapa plant possesses leaves which are entirely green (YGR) and are said to be non-variegated. Other plants have white or yellow blotches or streaks on their green leaves and are considered the variegated variety. In this experiment, you will investigate the inheritance of the variegated trait.

You will begin by constructing a Hypothesis for this trait that describes its mode of inheritance based on Mendelian Laws. You will be given variegated and non-variegated seeds which were harvested from parental plants that breed true for these phenotypes. You will plant these seeds, allow them to germinate, cross-pollinate the flowers, then harvest the F1 seeds to observe the phenotypic ratios of the F1 generation.

Before you plant seeds and begin this experiment, you must write a Protocol which states the hypothesis that you are testing and reveals the Prediction that you have formulated.

Key: You need not write two separate protocols. You can write one composite Protocol which differentiates between the two experimenets that are assigned, ie, their separate Hypotheses and Predictions must be stated. It is essential that in your Protocol you outline the cross for your Prediction and provide expected results in the form of a Punnett Square.

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